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Practical Enzymology
by Hans BisswangerA practice-oriented guide to assaying more than 100 of the most important enzymes, complete with the theoretical background and specific protocols for immediate use in the biochemical laboratory. Now expanded with a new section on metal ion determination.
Practical Enzymology
by Hans BisswangerThe second edition of the perfect companion for practical course work in enzymology. Now with an improved selection of enzymatic assays based on key metabolic reactions, this book provides the detailed background to the enzymatic reaction and the enzyme per se. Particular emphasis is placed on troubleshooting with the described methods, and, in addition, the new four-color layout throughout features detailed protocols highlighted for easy recognition. www.wiley-vch.de/home/enzymology: A companion website provides animations for all figures together with supplementary material, for deeper understanding of the partially abstract matter.
Practical Enzymology
by Hans BisswangerThe second edition of the perfect companion for practical course work in enzymology. Now with an improved selection of enzymatic assays based on key metabolic reactions, this book provides the detailed background to the enzymatic reaction and the enzyme per se. Particular emphasis is placed on troubleshooting with the described methods, and, in addition, the new four-color layout throughout features detailed protocols highlighted for easy recognition. www.wiley-vch.de/home/enzymology: A companion website provides animations for all figures together with supplementary material, for deeper understanding of the partially abstract matter.
Practical Fermentation Technology
by Brian McNeil Linda HarveyA hands-on book which begins by setting the context;- defining 'fermentation' and the possible uses of fermenters, and setting the scope for the book. It then proceeds in a methodical manner to cover the equipment for research scale fermentation labs, the different types of fermenters available, their uses and modes of operation. Once the lab is equipped, the issues of fermentation media, preservation strains and strain improvement strategies are documented, along with the use of mathematical modelling as a method for prediction and control. Broader questions such as scale-up and scale down, process monitoring and data logging and acquisition are discussed before separate chapters on animal cell culture systems and plant cell culture systems. The final chapter documents the way forward for fermenters and how they can be used for non-manufacturing purposes. A glossary of terms at the back of the book (along with a subject index) will prove invaluable for quick reference.Edited by academic consultants who have years of experience in fermentation technology, each chapter is authored by experts from both industry and academia. Industry authors come from GSK (UK), DSM (Netherlands), Eli Lilly (USA) and Broadley James (UK-USA).
Practical Field Ecology: A Project Guide
by C. Philip Wheater James R. Bell Penny A. CookThis book introduces experimental design and data analysis / interpretation as well as field monitoring skills for both plants and animals. Clearly structured throughout and written in a student-friendly manner, the main emphasis of the book concentrates on the techniques required to design a field based ecological survey and shows how to execute an appropriate sampling regime. The book evaluates appropriate methods, including the problems associated with various techniques and their inherent flaws (e.g. low sample sizes, large amount of field or laboratory work, high cost etc). This provides a resource base outlining details from the planning stage, into the field, guiding through sampling and finally through organism identification in the laboratory and computer based data analysis and interpretation. The text is divided into six distinct chapters. The first chapter covers planning, including health and safety together with information on a variety of statistical techniques for examining and analysing data. Following a chapter dealing with site characterisation and general aspects of species identification, subsequent chapters describe the techniques used to survey and census particular groups of organisms. The final chapter covers interpreting and presenting data and writing up the research. The emphasis here is on appropriate wording of interpretation and structure and content of the report.
Practical Field Ecology: A Project Guide
by C. Philip Wheater James R. Bell Penny A. CookThis book introduces experimental design and data analysis / interpretation as well as field monitoring skills for both plants and animals. Clearly structured throughout and written in a student-friendly manner, the main emphasis of the book concentrates on the techniques required to design a field based ecological survey and shows how to execute an appropriate sampling regime. The book evaluates appropriate methods, including the problems associated with various techniques and their inherent flaws (e.g. low sample sizes, large amount of field or laboratory work, high cost etc). This provides a resource base outlining details from the planning stage, into the field, guiding through sampling and finally through organism identification in the laboratory and computer based data analysis and interpretation. The text is divided into six distinct chapters. The first chapter covers planning, including health and safety together with information on a variety of statistical techniques for examining and analysing data. Following a chapter dealing with site characterisation and general aspects of species identification, subsequent chapters describe the techniques used to survey and census particular groups of organisms. The final chapter covers interpreting and presenting data and writing up the research. The emphasis here is on appropriate wording of interpretation and structure and content of the report.
Practical Field Ecology: A Project Guide
by C. Philip Wheater James R. Bell Penny A. CookOffers a comprehensive, accessible introduction to experimental design, field monitoring skills for plants and animals, data analysis, interpretation and reporting This user-friendly book presents field monitoring skills for both plants and animals, within the context of a research project. This text provides a single resource to take the reader all the way through from the planning stage, into the field, guiding through sampling, organism identification, computer-based data analysis and interpretation, and finally how to present the results to maximise the impact of the work. Logically structured throughout, and revised extensively in the second edition, the book concentrates on the techniques required to design a field-based ecological survey and shows how to execute an appropriate sampling regime. It evaluates appropriate sampling and analytical methods, identifying potential problems associated with various techniques and how to mitigate these. The second edition of this popular text has updated reference material and weblinks, increased the number of case studies by 50% to illustrate the use of specific techniques in the field, added over 20% more figures (including 8 colour plates), and made more extensive use of footnotes to provide extra details. Extensions to topics covered in the first edition include additional discussion of: ethical issues; statistical methods (sample size estimation, use of the statistical package R, mixed models); bioindicators, especially for freshwater pollution; seeds, fecundity and population dynamics including static and dynamic life tables; forestry techniques including tree coring and tree mortality calculations; the use of data repositories; writing for a journal and producing poster and oral presentations. In addition, the use of new and emerging technologies has been a particular focus, including mobile apps for environmental monitoring and identification; land cover and GIS; the use of drones including legal frameworks and codes of practice; molecular field techniques including DNA analysis in the field (including eDNA); photo-matching for identifying individuals; camera trapping; modern techniques for detecting and analysing bat echolocation calls; and data storage using the cloud. Divided into six distinct chapters, Practical Field Ecology, 2nd Edition begins at project inception with a chapter on planning—covering health and safety, along with guidance on how to ensure that the sampling and experimental design is suitable for subsequent statistical analysis. Following a chapter dealing with site characterisation and general aspects of species identification, subsequent chapters describe the techniques used to survey and census particular groups of organisms. The final chapters cover analysing, interpreting and presenting data, and writing up the research. Offers a readable and approachable integrated guide devoted to field-based research projects Takes students from the planning stage, into the field, and clearly guides them through organism identification in the laboratory and computer-based data analysis, interpretation and data presentation Includes a chapter on how to write project reports and present findings in a variety of formats to differing audiences Aimed at undergraduates taking courses in Ecology, Biology, Geography, and Environmental Science, Practical Field Ecology, 2nd Edition will also benefit postgraduates seeking to support their projects.
Practical Field Ecology: A Project Guide
by C. Philip Wheater James R. Bell Penny A. CookOffers a comprehensive, accessible introduction to experimental design, field monitoring skills for plants and animals, data analysis, interpretation and reporting This user-friendly book presents field monitoring skills for both plants and animals, within the context of a research project. This text provides a single resource to take the reader all the way through from the planning stage, into the field, guiding through sampling, organism identification, computer-based data analysis and interpretation, and finally how to present the results to maximise the impact of the work. Logically structured throughout, and revised extensively in the second edition, the book concentrates on the techniques required to design a field-based ecological survey and shows how to execute an appropriate sampling regime. It evaluates appropriate sampling and analytical methods, identifying potential problems associated with various techniques and how to mitigate these. The second edition of this popular text has updated reference material and weblinks, increased the number of case studies by 50% to illustrate the use of specific techniques in the field, added over 20% more figures (including 8 colour plates), and made more extensive use of footnotes to provide extra details. Extensions to topics covered in the first edition include additional discussion of: ethical issues; statistical methods (sample size estimation, use of the statistical package R, mixed models); bioindicators, especially for freshwater pollution; seeds, fecundity and population dynamics including static and dynamic life tables; forestry techniques including tree coring and tree mortality calculations; the use of data repositories; writing for a journal and producing poster and oral presentations. In addition, the use of new and emerging technologies has been a particular focus, including mobile apps for environmental monitoring and identification; land cover and GIS; the use of drones including legal frameworks and codes of practice; molecular field techniques including DNA analysis in the field (including eDNA); photo-matching for identifying individuals; camera trapping; modern techniques for detecting and analysing bat echolocation calls; and data storage using the cloud. Divided into six distinct chapters, Practical Field Ecology, 2nd Edition begins at project inception with a chapter on planning—covering health and safety, along with guidance on how to ensure that the sampling and experimental design is suitable for subsequent statistical analysis. Following a chapter dealing with site characterisation and general aspects of species identification, subsequent chapters describe the techniques used to survey and census particular groups of organisms. The final chapters cover analysing, interpreting and presenting data, and writing up the research. Offers a readable and approachable integrated guide devoted to field-based research projects Takes students from the planning stage, into the field, and clearly guides them through organism identification in the laboratory and computer-based data analysis, interpretation and data presentation Includes a chapter on how to write project reports and present findings in a variety of formats to differing audiences Aimed at undergraduates taking courses in Ecology, Biology, Geography, and Environmental Science, Practical Field Ecology, 2nd Edition will also benefit postgraduates seeking to support their projects.
Practical Fire and Arson Investigation
by David R. Redsicker John J. O'ConnorDid you know. . .. . . that arson has been described as the fastest-growing crime in America?. . . that arson is the most expensive crime committed?. . . that over 8 billion dollars was estimated lost due to fires in 1994?. . . that an estimated 86,000 structure fires of incendiary or suspicious origin were reported in 1994?Davi
Practical Flow Cytometry
by Howard M. ShapiroFrom the reviews of the 3rd Edition... "The standard reference for anyone interested in understanding flow cytometry technology." American Journal of Clinical Oncology "...one of the most valuable of its genre and...addressed to a wide audience?written in such an attractive way, being both informative and stimulating." Trends in Cell Biology This reference explains the science and discusses the vast biomedical applications of quantitative analytical cytology using laser-activated detection and cell sorting. Now in its fourth edition, this text has been expanded to provide full coverage of the broad spectrum of applications in molecular biology and biotechnology today. New to this edition are chapters on automated analysis of array technologies, compensation, high-speed sorting, reporter molecules, and multiplex and apoptosis assays, along with fully updated and revised references and a list of suppliers.
Practical Fluorescence Spectroscopy
by Zygmunt (Karol) Gryczynski Ignacy GryczynskiPresenting a detailed, hands-on approach to fluorescence spectroscopy, this book describes experiments that cover basic spectroscopy and advanced aspects of fluorescence spectroscopy. It emphasizes practical guidance, providing background on fundamental concepts as well as guidance on how to handle artifacts, avoid common errors, and interpret data. Nearly 150 experiments from biophysics, biochemistry, and the biomedical sciences demonstrate how methods are applied in practical applications. The result is a hands-on guide to the most important aspects of fluorescence spectroscopy, from steady-state fluorescence to advanced time-resolved fluorescence. Provides a complete overview of nearly 150 experiments using fluorescence spectroscopy, from basic to advanced applications Presents laboratory methods using a variety of instrumental setups with detailed discussion of data analysis and interpretations Covers steady-state phenomena, time-resolved phenomena, and advanced methods Spans biophysical, biochemical, and biomedical applications Describes related concepts, theory, and mathematical background as well as commercially available instruments used for measurements
Practical Fluorescence Spectroscopy
by Zygmunt (Karol) Gryczynski Ignacy GryczynskiPresenting a detailed, hands-on approach to fluorescence spectroscopy, this book describes experiments that cover basic spectroscopy and advanced aspects of fluorescence spectroscopy. It emphasizes practical guidance, providing background on fundamental concepts as well as guidance on how to handle artifacts, avoid common errors, and interpret data. Nearly 150 experiments from biophysics, biochemistry, and the biomedical sciences demonstrate how methods are applied in practical applications. The result is a hands-on guide to the most important aspects of fluorescence spectroscopy, from steady-state fluorescence to advanced time-resolved fluorescence. Provides a complete overview of nearly 150 experiments using fluorescence spectroscopy, from basic to advanced applications Presents laboratory methods using a variety of instrumental setups with detailed discussion of data analysis and interpretations Covers steady-state phenomena, time-resolved phenomena, and advanced methods Spans biophysical, biochemical, and biomedical applications Describes related concepts, theory, and mathematical background as well as commercially available instruments used for measurements
Practical Forensic Digital Imaging: Applications and Techniques (Practical Aspects Of Criminal And Forensic Investigations Ser.)
by Patrick JonesWell-documented scenes can prove to be invaluable pieces of evidence at trial, and the ability to take compelling photographs is a critical skill for forensic scientists and investigators. Practical Forensic Digital Imaging: Applications and Techniques is an up-to-date and thorough treatment of digital imaging in the forensic sciences. Balancing pr
Practical Forensic Microscopy: A Laboratory Manual
by Barbara P. WheelerAn applied approach to teaching forensic microscopy in educational settings, featuring new experiments and an up-to-date overview of the field Practical Forensic Microscopy: A Laboratory Manual, 2nd Edition, is a unique resource that brings the microscopic procedures used by real-world forensic investigators to the college laboratory, providing hands-on knowledge of the microscopes and microscopic techniques used in the field. Presenting a balanced, skills-based approach to the subject, this student-friendly lab manual contains dozens of experiments designed to cover the various microscopic evidence disciplines, including examinations of fingerprints, firearm, toolmark, shoeprint and tire impressions, gunshots, fibers, soil, glass breakage, drugs, semen, and human hair. The second edition includes revised and updated experiments that reflect current technologies and techniques used in forensic science, including new experiments examining plastic film, food condiments, feathers, building materials, explosive residue, cigarette butts and more. Each chapter includes a list of simple objectives for the experiment, a general overview of the topic, further readings, and selected references. The manual contains worksheets and templates for students to use when compiling analytical results. The concluding chapter features an innovative case scenario that requires students to analyze items of evidence, complete a laboratory report, reach a conclusion, and present their findings. This popular lab manual: Teaches practical forensic microscopy skills through hands-on experiments and engaging practical activities Covers a wide range of microscopes and forensic tools, including stereomicroscopes, ocular micrometers, and fluorescence, polarized light, and phase contrast microscopes Explains simple stereomicroscopic techniques for analyzing various types of common forensic evidence Includes more complex procedures for examining biological, drug, and trace evidence Discusses laboratory safety, microscope maintenance, and the Micro Kit Written by an author with years of academic and professional experience, Practical Forensic Microscopy: A Laboratory Manual, 2nd Edition, is a must-have companion for any college-level forensic science course with a laboratory component, and is a useful supplement for related courses that cover microscopy and the principles of forensic lab procedures.
Practical Forensic Microscopy: A Laboratory Manual
by Barbara P. WheelerAn applied approach to teaching forensic microscopy in educational settings, featuring new experiments and an up-to-date overview of the field Practical Forensic Microscopy: A Laboratory Manual, 2nd Edition, is a unique resource that brings the microscopic procedures used by real-world forensic investigators to the college laboratory, providing hands-on knowledge of the microscopes and microscopic techniques used in the field. Presenting a balanced, skills-based approach to the subject, this student-friendly lab manual contains dozens of experiments designed to cover the various microscopic evidence disciplines, including examinations of fingerprints, firearm, toolmark, shoeprint and tire impressions, gunshots, fibers, soil, glass breakage, drugs, semen, and human hair. The second edition includes revised and updated experiments that reflect current technologies and techniques used in forensic science, including new experiments examining plastic film, food condiments, feathers, building materials, explosive residue, cigarette butts and more. Each chapter includes a list of simple objectives for the experiment, a general overview of the topic, further readings, and selected references. The manual contains worksheets and templates for students to use when compiling analytical results. The concluding chapter features an innovative case scenario that requires students to analyze items of evidence, complete a laboratory report, reach a conclusion, and present their findings. This popular lab manual: Teaches practical forensic microscopy skills through hands-on experiments and engaging practical activities Covers a wide range of microscopes and forensic tools, including stereomicroscopes, ocular micrometers, and fluorescence, polarized light, and phase contrast microscopes Explains simple stereomicroscopic techniques for analyzing various types of common forensic evidence Includes more complex procedures for examining biological, drug, and trace evidence Discusses laboratory safety, microscope maintenance, and the Micro Kit Written by an author with years of academic and professional experience, Practical Forensic Microscopy: A Laboratory Manual, 2nd Edition, is a must-have companion for any college-level forensic science course with a laboratory component, and is a useful supplement for related courses that cover microscopy and the principles of forensic lab procedures.
Practical Forensic Microscopy: A Laboratory Manual
by Barbara Wheeler Lori J. WilsonForensic Microscopy: A Laboratory Manual will provide the student with a practical overview and understanding of the various microscopes and microscopic techniques employed within the field of forensic science. Each laboratory experiment has been carefully designed to cover the variety of evidence disciplines within the forensic science field with carefully set out objectives, explanations of each topic and worksheets to help students compile and analyse their results. The emphasis is placed on the practical aspects of the analysis to enrich student understanding through hands on experience. The experiments move from basic through to specialised and have been developed to cover a variety of evidence disciplines within forensic science field. The emphasis is placed on techniques currently used by trace examiners. This unique, forensic focused, microscopy laboratory manual provides objectives for each topic covered with experiments designed to reinforce what has been learnt along with end of chapter questions, report requirements and numerous references for further reading. Impression evidence such as fingerprints, shoe tread patterns, tool marks and firearms will be analysed using simple stereomicroscopic techniques. Body fluids drug and trace evidence (e.g. paint glass hair fibre) will be covered by a variety of microscopes and specialized microscopic techniques.
Practical Forensic Microscopy: A Laboratory Manual
by Barbara Wheeler Lori J. WilsonForensic Microscopy: A Laboratory Manual will provide the student with a practical overview and understanding of the various microscopes and microscopic techniques employed within the field of forensic science. Each laboratory experiment has been carefully designed to cover the variety of evidence disciplines within the forensic science field with carefully set out objectives, explanations of each topic and worksheets to help students compile and analyse their results. The emphasis is placed on the practical aspects of the analysis to enrich student understanding through hands on experience. The experiments move from basic through to specialised and have been developed to cover a variety of evidence disciplines within forensic science field. The emphasis is placed on techniques currently used by trace examiners. This unique, forensic focused, microscopy laboratory manual provides objectives for each topic covered with experiments designed to reinforce what has been learnt along with end of chapter questions, report requirements and numerous references for further reading. Impression evidence such as fingerprints, shoe tread patterns, tool marks and firearms will be analysed using simple stereomicroscopic techniques. Body fluids drug and trace evidence (e.g. paint glass hair fibre) will be covered by a variety of microscopes and specialized microscopic techniques.
Practical Forensic Psychiatry
by Tom Clark Dharjinder Singh RoopraiSuccinct, structured, and focused, this book concentrates on the key facts and practical day-to-day issues vital to forensic psychiatry.Includes fact-oriented practical advice and clinically relevant examples Reflects current practice and the latest laws Provides tips on testifying in legal mattersFeatures a clear bullet point style to help readers
Practical Fracture Mechanics in Design
by Arun ShuklaTheoretical treatments of fracture mechanics abound in the literature. Among the first books to address this vital topic from an applied standpoint was the first edition of Practical Fracture Mechanics in Design. Completely updated and expanded to reflect recent developments in the field, the second edition of this valuable reference concisely revi
Practical Fruits of Econophysics: Proceedings of The Third Nikkei Econophysics Symposium
by Hideki TakayasuSome economic phenomena are predictable and controllable, and some are impos sible to foresee. Existing economic theories do not provide satisfactory answers as to what degree economic phenomena can be predicted and controlled, and in what situations. Against this background, people working on the financial front lines in real life have to rely on empirical rules based on experiments that often lack a solid foundation. "Econophysics" is a new science that analyzes economic phenomena empirically from a physical point of view, and it is being studied mainly to offer scientific, objective and significant answers to such problems. This book is the proceedings of the third Nikkei symposium on ''Practical Fruits of Econophysics," held in Tokyo, November 9-11, 2004. In the first symposium held in 2000, empirical rules were established by analyzing high-frequency finan cial data, and various kinds of theoretical approaches were confimied. In the second symposium, in 2002, the predictability of imperfections and of economic fluctua tions was discussed in detail, and methods for applying such studies were reported. The third symposium gave an overview of practical developments that can immedi ately be applied to the financial sector, or at least provide hints as to how to use the methodology.
Practical Functional Group Synthesis
by Robert A. Stockland Jr.A practical handbook for chemists performing bond forming reactions, this book features useful information on the synthesis of common functional groups in organic chemistry. • Details modern functional group synthesis through carbon-heteroelement (N, O, P, S, B, halogen) bond forming reactions with a focus on operational simplicity and sustainability. • Summarizes key and recent developments – which are otherwise scattered across journal literature – into a single source• Contains over 100 detailed preparations of common functional groups• Included 25 troubleshooting guides with suggestions and potential solutions to common problems.• Complements the text in enhanced ebook editions with tutorial videos where the author provides an introduction to microwave assisted chemistry
Practical Functional Group Synthesis
by Robert A. Stockland Jr.A practical handbook for chemists performing bond forming reactions, this book features useful information on the synthesis of common functional groups in organic chemistry. • Details modern functional group synthesis through carbon-heteroelement (N, O, P, S, B, halogen) bond forming reactions with a focus on operational simplicity and sustainability. • Summarizes key and recent developments – which are otherwise scattered across journal literature – into a single source• Contains over 100 detailed preparations of common functional groups• Included 25 troubleshooting guides with suggestions and potential solutions to common problems.• Complements the text in enhanced ebook editions with tutorial videos where the author provides an introduction to microwave assisted chemistry
Practical Gamma-ray Spectroscopy
by Gordon GilmoreThe Second Edition of Practical Gamma-Ray Spectrometry has been completely revised and updated, providing comprehensive coverage of the whole gamma-ray detection and spectrum analysis processes. Drawn on many years of teaching experience to produce this uniquely practical volume, issues discussed include the origin of gamma-rays and the issue of quality assurance in gamma-ray spectrometry. This new edition also covers the analysis of decommissioned nuclear plants, computer modelling systems for calibration, uncertainty measurements in QA, and many more topics.
Practical Gamma-ray Spectroscopy
by Gordon GilmoreThe Second Edition of Practical Gamma-Ray Spectrometry has been completely revised and updated, providing comprehensive coverage of the whole gamma-ray detection and spectrum analysis processes. Drawn on many years of teaching experience to produce this uniquely practical volume, issues discussed include the origin of gamma-rays and the issue of quality assurance in gamma-ray spectrometry. This new edition also covers the analysis of decommissioned nuclear plants, computer modelling systems for calibration, uncertainty measurements in QA, and many more topics.
Practical Gas Chromatography: A Comprehensive Reference
by Katja Dettmer-Wilde Werner EngewaldGas chromatography continues to be one of the most widely used analytical techniques, since its applications today expand into fields such as biomarker research or metabolomics. This new practical textbook enables the reader to make full use of gas chromatography. Essential fundamentals and their implications for the practical work at the instrument are provided, as well as details on the instrumentation such as inlet systems, columns and detectors. Specialized techniques from all aspects of GC are introduced ranging from sample preparation, solvent-free injection techniques, and pyrolysis GC, to separation including fast GC and comprehensive GCxGC and finally detection, such as GC-MS and element-specific detection. Various fields of application such as enantiomer, food, flavor and fragrance analysis, physicochemical measurements, forensic toxicology, and clinical analysis are discussed as well as cutting-edge application in metabolomics is covered.